If your blood contains antibodies to the antigen, the two will bind together. In the lab, a technician will add the sample to a petri dish containing the specific antigen related to the condition for which you are being tested. The blood sample will be sent to a laboratory for analysis. This procedure should be relatively painless, but your arm may throb a little after it’s done. You’ll be asked to maintain pressure at the site where the needle was inserted for a few minutes to reduce blood flow. When enough blood has been collected, the needle will be removed and a small bandage will be placed on your arm where the needle was. Next, a needle will be placed in one of your veins to draw a small sample of blood. Then, a tourniquet, or band, will be applied around your arm to create pressure and cause your veins to swell with blood. First, a healthcare provider will cleanse your arm with an antiseptic. The ELISA test involves taking a sample of your blood. You’ll probably need to sign a consent form, and your doctor should explain the reason for doing the test. cdc.gov/hiv/pdf/guidelines_testing_recommendedlabtestingalgorithm.The ELISA test is simple and straightforward.
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